Last data update: 24 January 2024 16:39 CET
Host name: Escherichia coli B Evo21(DE3) (LMBP 12941)
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| Species: | Escherichia coli |
| Group: | B |
| Strain designation: | Evo21(DE3) |
| Accession number: | LMBP 12941 |
| Depositor: | Dr S. Heyde1 and Prof. Dr M. Nørholm1. It was constructed by Dr S. Heyde1. 1 The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Horsholm, Denmark |
| Containment level: | This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification). |
| Medium: | LB-Lennox |
| Selection marker: | - |
| Temperature: | 28°C |
| Genotype: | F- ompT gal dcm lon hsdSB(rB- mB-) λ(DE3 [lacI lacUV5-T7gene1 ind1 sam7 nin5]) ΔlacI ΔlacZ rne::IS1 |
| Phenotype: | - |
| Properties: | This strain is intended for protein production. The strain contains DE3, a λ prophage carrying the T7 RNA polymerase gene under control of the lac UV5 promoter and lacIq. The lacI and lacZ genes have been deleted. |
| Further information: | The genome sequence of this strain corresponds with EMBL project number PRJNA741995. |
| Original reference: | Heyde et al., Commun. Biol. 4 (2021), 963 [PMID: 34385596] [DOI: 10.1038/s42003-021-02493-4] |
| Restricted use: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department. |
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Refer in your Materials and Methods: |
Escherichia coli B Evo21(DE3) (LMBP 12941) is available at BCCM/GeneCorner. It was deposited by Dr S. Heyde and Prof. Dr M. Nørholm and was published in Heyde et al., 2021. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.